China Urine Analyzer Suppliers
Urine Analyzer is an automated instrument for determining certain chemical components in urine. It is an important tool for automated urine inspection in medical laboratories. This instrument has the advantages of simple and fast operation. However, improper use of urine analyzers and many intermediate links and influencing factors directly affect the accuracy of automated analysis results, which will not only cause errors in experimental results, but even delay diagnosis. Therefore, operators are required to understand the principles, performance and precautions of automated instruments. And the knowledge of influencing factors and other aspects are fully understood, and the correct use of automated instruments can make the results obtained by the urine analyzer more reliable and accurate.
application
In the 1950s, a single dry chemical test strip method was used to measure protein and glucose in urine, and the changes in the color of the test strip were observed with the naked eye and compared with the standard plate to obtain the corresponding values. In the 1980s, due to the high development and widespread use of computer technology, automated urine analyzers also developed rapidly, gradually developing from semi-automatic to fully-automated. Urine analyzers are often divided into two categories according to the test items: ① 8-11 screening combined urine test strips mainly used for newly diagnosed patients and health examinations. The eight test items included protein, glucose, PH, ketone bodies, bilirubin, urobilinogen, red blood cells (occult blood) and nitrite; in addition to the above eight tests, urine leukocyte test was added to the nine test items. On the basis of 9 of the 10 urine analyzer testing items, the urine specific density test was added. 11 testing items have added vitamin C testing. ②It is mainly used for the observation of the curative effect of the diagnosed diseases, such as the combination test strip of PH, protein and occult blood (red blood cells) for kidney disease; the combination test strip of PH, sugar and ketone body for diabetes; the combination of bilirubin and urobilinogen for liver disease test tape.
principle
This type of instrument is generally controlled by a microcomputer, and the color change on the test strip is measured semi-quantitatively by using a spherical integrator to receive dual-wavelength reflected light. There are several reagent pads containing various reagents on the reagent strip, each of which reacts independently with the corresponding components in the urine, and displays different colors. The depth of the color is proportional to a certain component in the urine, and there is another in the reagent strip" Compensation pad", as the urine background color, compensates for the errors caused by colored urine and instrument changes.
Put the reagent strip with urine adsorbed in the colorimetric tank of the instrument, and the various reagent pads that have produced chemical reactions on the reagent strip are illuminated by the light source, and the reflected light is received by the spherical analyzer, and the photocell of the spherical analyzer is reflected. Irradiate with dual-wavelength light (measurement light passing through the filter and a reference light), and the selection of each wavelength is determined by the detection item.
The instrument automatically calculates the reflectance according to the following formula, and then compares it with the standard curve, and automatically finds and prints the corresponding results of various components. If the content of a certain component in the urine is high, the reflected light of the corresponding reagent pad is dark, otherwise it is strong.
Reflectance fraction: R(%)=Tm.Cs/TsCm×100%
In the formula, R(%) is the reflectivity; Tm is the reflection intensity of the reagent pad to the measurement wavelength; Ts is the reflection intensity of the reagent pad to the reference wavelength; Cm is the reflection intensity of the calibration pad to the measurement defect length; Cs is the calibration pair. Reflection intensity at the reference wavelength.
